Composite

Part:BBa_K1487021:Design

Designed by: Samantha Bermudez   Group: iGEM14_UT-Dallas   (2014-10-02)


Promoter + Guide RNA (gRNA) target for acfA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Target sequence must be 20 base pairs and be flanked with a PAM sequence (NGG) on the 3' end in order for Cas9 + gRNA to successfully target and cleave the sequence.


Source

Part was assembled using complementary primers to match a 20 base pair sequence in the acfA coding region.

References